(P11-038-25) Relationship Between Dietary Inflammatory Index, Inflammatory Markers, Microbiota, and Risk of Anxiety and Depression in Young Individuals With Obesity: A Study Protocol

Objectives: To evaluate the relationship between the Dietary Inflammatory Index (DII), levels of inflammatory markers, gut microbiota composition, and the risk of anxiety and depression in young individuals with obesity.

Hypothesis: A pro-inflammatory diet will be significantly associated with elevated levels of inflammatory markers, alterations in the composition of the gut microbiota, and an increased risk of anxiety and depression in young individuals with obesity.

Methods: A cross-sectional observational study will be conducted. Dietary patterns will be analyzed using a validated semi-quantitative food frequency questionnaire to calculate DII scores. Inflammatory markers such as C-reactive protein (CRP), MCP-1, IL6, IL1β, and IL10 will be measured through ProQuantum^TM immunoassays. Gut microbiota composition will be assessed using sequencing techniques. Anxiety and depression symptoms will be evaluated through validated clinical scales. Multivariate statistical models will be applied to explore associations among variables.

Results: N/A

Conclusions: This study is anticipated to provide robust evidence linking pro-inflammatory dietary patterns to elevated inflammatory markers and gut microbiota alterations, highlighting the gut-brain axis as a critical pathway influencing mental and metabolic health in young individuals with obesity. Findings are expected to support the development of targeted dietary and microbiota-based interventions as non-invasive strategies to mitigate inflammation and improve mental and physical health outcomes, offering significant implications for public health policies and clinical practices to reduce obesity-related comorbidities.

Funding Sources: This study will be funded by the Jesuit University System in Mexico.