PROFESSOR University of Florida Gainesville, Florida, United States
Disclosure(s):
Shuhan Li, Master: No relevant financial relationship(s) with ineligible companies to disclose.
Objectives: The study aimed to screen for UTI-susceptible women and evaluate their responses to cranberry juice based on their post-consumption urinary activities to inhibit P-type uropathogenic E. coli adhesion.
Methods: Healthy women (n = 150) consumed 10 oz of cranberry juice daily for 4 days after a 10-day run-in in a screening trial. Morning urine was collected at baseline and post-consumption. Urinary anti-adhesion activities were measured by a fluorometric method and expressed in anti-adhesion index (AAI). After screening, selected participants were given 10 oz of 100% cranberry juice or light apple juice daily for 3 weeks after a 10-day run-in, then switched juices following a 3-week washout. Morning urine was collected on days 0, 5, and 22 after each drinking period. AAI changes were analyzed using mixed effect models (trial registration NCT05065008).
Results: Seventy-six out of 150 women had baseline AAI <131 and were classified as UTI-susceptible. Twenty-six and 50 susceptible women were classified as positive responders and non-positive responders, respectively, based on changes in post-consumption AAIs. After screening, 15 positive and 15 non-positive responders were selected for the cross-over trial. The AAIs (n = 30) increased significantly after cranberry juice intake than apple juice intake (p < 0.05). Positive responders showed significantly higher AAIs after cranberry juice intake, while no significant differences were observed in non-positive responders. Mixed effect models (n = 15) were run separately on positive and non-positive responders, showing consistent results that the cranberry intake significantly improved the AAIs of positive responders but not non-positive responders. A lack of time effects in the model suggested a similar improvement of AAI after 4 days and 21 days of cranberry juice consumption.
Conclusions: UTI-susceptible women responded differently to cranberry juice intake. The group of 15 positive responders from the screening showed significantly higher AAIs after the intake of cranberry juice than apple juice in the cross-over trial. Non-positive responders showed no significant differences.
Funding Sources: National Institute of Food and Agriculture (grant 2022-67017-36240)
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