Skip to main content
NUTRITION 2025 Main banner

Obesity (Poster Session)

(P20-065-25) Dietary Omega-3 Polyunsaturated Fatty Acids Attenuate Obesity and Associated Disorder in Mice: Potential Importance of Cytochrome P450-Derived Omega-3 Fatty Acid Epoxides

Sunday, June 1, 2025
11:45 AM - 12:45 PM ET
Location: Poster Board D-52

    Presenting Author(s)

  • Yige Wang, graduate student

    Ph.D student
    University of California, Davis
    Davis, California, United States

    • Co-Author(s)

  • GZ

    Guodong Zhang

    University of California, Davis, United States

  • Nan Jing, PhD

    Postdoctoral researcher
    University of California, Davis
    Davis, California, United States

Disclosure(s):
Yige Wang, graduate student: No relevant financial relationship(s) with ineligible companies to disclose.
Nan Jing, PhD: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: Human and preclinical studies have shown that a high intake of omega-3 polyunsaturated fatty acids (PUFAs) is associated with a reduced risk of obesity and related disorders. However, the underlying mechanism remain poorly understood. The objective of this study was to investigate the effects and mechanisms of dietary omega-3 PUFAs on obesity.

Methods: We treated C57BL/6 mice with a low-fat diet, a high-fat diet, or omega-3 PUFA-enriched high-fat diets, then the development of obesity, adipose inflammation and dysfunction, and other metabolic disorders were studied. In addition, we used LC-MS/MS-based lipidomics, which can analyze >100 lipid metabolites, to study how omega-3 PUFA intake modulates obesity-associated lipid metabolism.

Results: Dietary administration of omega-3 PUFA reduced obesity development in a dose-dependent manner, illustrating the anti-obese effects of omega-3 PUFAs. LC-MS/MS lipidomics showed that omega-3 intake significantly altered the profiles of lipid metabolites in tissues, with cytochrome P450-derived omega-3 fatty acid epoxides showing the most pronounced increase.

Conclusions: Our studies support the anti-obese effects of dietary omega-3 PUFAs. Furthermore, the lipidomics analysis suggests that omega-3 epoxides may play a key role in the health benefits of omega-3 PUFAs.

Funding Sources: This research is supported by a new faculty start-up grant from the University of California Davis (to G.Z.).