(EA02-16-25) Acute and Chronic Intake of Berry Polyphenols and Fructo-oligosaccharide on Bile Acid Composition in Human Plasma

Scientific conference where the work was recently presented: International Conference on Polyphenols and Health 2024

Date on which the work was previously presented at the scientific conference listed: October 16th-19th, 2024

Objectives: The objective of this study was to assess the effects of acute and chronic intake of strawberries and red raspberries on bile acid composition in adults with low-grade inflammation, and to evaluate potential additive effects by combining a pre-biotic (fructo-oligosaccharide) with these berry polyphenols.

Methods: In this 4-week randomized, parallel, single-blinded, placebo-controlled study, 92 participants (35 ± 13 years, 29.8 ± 4.5 kg/m2; mean ± SD) consumed one of four beverages as part of their daily diet: freeze-dried strawberry and red raspberry composite (SBRR), fructo-oligosaccharide (FOS), SBRR + FOS or a control (placebo devoid of polyphenols). Fasting blood samples were collected at baseline (Day 0), 24 hours after a single intake (Day 1) and at the end of study (Day 28). Bile acid composition in plasma was assessed using ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometer (UHPLC-QQQ-MS). Statistical analyses were performed using paired t-tests for within intervention comparisons and ANCOVA for between intervention comparisons using SAS 9.4 (SAS Institute, Inc., Cary, NC, USA).

Results: A total of 134 bile acids were quantified in plasma samples. Twelve bile acids were included for the purpose of this analysis. Data processing for remaining bile acids is still underway. Preliminary analysis showed that a single intake of FOS significantly reduced plasma concentrations of murideoxycholic acid (MuriDCA) after 24 hours (Day 0: 205.0 ± 119.0 nmol/L, Day 1: 95.7 ± 27.7 nmol/L; p=0.050). At Day 28, plasma concentrations of deoxycholic acid (DCA) were significantly lower in the SBRR + FOS (174.3 ± 35.0 nmol/L) group compared to SBRR (293.4 ± 41.9 nmol/L; p=0.020) and control (289.5 ± 78.9 nmol/L; p=0.012).

Conclusions: Incorporating FOS and SBRR+FOS into the diet significantly alters select secondary bile acid concentrations in plasma after acute and chronic intake.

Funding Sources: This work is supported by Agricultural and Food Research Initiative {grant no. 2019-67017-29254/project accession no. 1018625} from the USDA National Institute of Food and Agriculture.