Founder Microgenesis Co San Francisco, California, United States
Disclosure(s):
Gabriela Gutierrez, PhD: Microgenesis Co: Executive Role (e.g. Board of Directors) (Ongoing)
Objectives: To investigate the relationship between nutritional lifestyle factors, gut microbiota, and reproductive health in women, using microRNAs (miRNAs) as biomarkers for personalized dietary interventions.
Methods: This analytical, prospective study (2018-2023) included 420 women (20 fertile, 400 infertile; 48 USA, 100 Spain, 252 Argentina) with 0 parity, 2.5 failed IVF cycles, and 4.2 years. Biomarker analysis included saliva (sIgA), blood (insulin, LDL, TPO, ANA), vaginal and anal swabs (DNA microbiota profiling, miR-21, miR-155 via qPCR).
Results: No altered biomarkers were found in fertile women. Among infertile women:
31% had low sIgA
23% had hypercholesterolemia (LDL)
18% tested positive for TPO/ANA
13% had hyperinsulinemia
BMI: 23.6
Women with increased miR-21 & miR-155 had reduced bacterial diversity, lower Bacteroidetes, and higher Firmicutes, increasing the Firmicutes/Bacteroidetes ratio. miR-21 overexpression correlated with elevated Lactobacillus (p < 0.05).
miR-21: Linked to tight junction disruption, autoimmunity, M2 macrophage polarization, fungal overgrowth, and bacterial depletion.
miR-155: Associated with inflammation, M1 macrophage activation, endometriosis, and Gram-negative bacterial overgrowth.
Personalized anti-inflammatory diets were designed based on inflammatory profiles, targeting IgA deficiency, metabolic inflammation, gut dysbiosis, insulin resistance, microbial diversity, oxidative stress, and immune activation.
With tailored nutraceuticals and dietary plans, live birth rates increased from 0% to 60% within 90 days.
Conclusions: miRNAs (miR-21 & miR-155) serve as key biomarkers in reproductive health. Their integration with microbiota profiling allows personalized dietary strategies to improve fertility outcomes.
Funding Sources: This study was supported by institutional grants and private funding for reproductive health research.
