(P26-057-25) AI-Generated Protein Combinations With High Biosimilarity: A Novel Approach to Addressing Gut Health and Inflammation

Objectives: To evaluate the anti-inflammatory efficacy of an AI-generated protein combination with high biosimilarity to human breast milk proteins, derived from bovine colostrum.

Methods: Ex-vivo experiments used human peripheral blood mononuclear cells (PBMCs) activated with OKT3 to mimic inflammation. The AI-generated protein combination's effects on T-cell activation and INF-γ secretion were compared to colostrum powder, lactoferrin, and curcumin. In-vivo efficacy was assessed using a delayed-type hypersensitivity (DTH) mouse model induced by oxazolone.

Results: The AI-generated protein combination significantly reduced T-cell activation and IFN-γ secretion in vitro. In vivo, mice treated with the combination exhibited a 39% reduction in ear thickness after 48 hours, compared to 29% in untreated controls.

Conclusions: This study demonstrates that AI-generated protein combinations with high biosimilarity offer a robust solution for managing gut inflammation and promoting immune health. This formulation provides a promising foundation for developing functional nutrition products targeting chronic inflammation and gut health disorders.

Funding Sources: The Israel Innovation Authority