(P22-048-25) A Randomized Crossover-Controlled Trial Investigating the Epigenetic Impact of a Greens-Based Supplement in Adults

Objectives: The objective of this study is to evaluate the effects of a 30-day greens-based supplement on epigenetic markers of aging and metabolic health in adults aged 50–65 years with a BMI >30 kg/m². We hypothesize that consuming 10 g/day of the supplement for 30 days will result in significant improvements in epigenetic aging profiles compared to the control period.

Methods: This 60-day randomized crossover clinical trial (RCT) evaluated the effects of a greens-based supplement in adults aged 50–65 years with low dark green leafy vegetable (DGLV) intake ( < 1 serving per week) and no overt medical conditions. Participants (n=20, 70% female, mean age 57 ± 5 years, BMI 38.1 ± 8) were randomized to immediate or delayed intervention groups and all participants wore and synced Garmin Vivofit4 devices for the duration of the study. During the 30-day intervention, participants consumed the supplement each morning and confirmed adherence via text message. Study visits included assessments of body composition via bioelectrical impedance, fasting blood draws, and completion of medical history, physical activity, and health-related quality of life questionnaires. A multivariate ANOVA will be performed to evaluate primary and secondary outcomes, with post hoc repeated measures ANOVA and paired-samples t-tests for pre- and post-intervention comparisons. Spearman correlations will assess relationships among variables, with significance set at p < 0.05.

Results: N/A

Conclusions: This will be the first RCT to evaluate the effects of a greens-based supplement on DNA methylation in peripheral blood mononuclear cells and the fecal microbiome. Secondary outcomes include fasting triglycerides, low density lipoproteins, blood glucose, visceral and total body fat percentage, breath hydrogen and methane levels, actigraphy, total caloric intake (via 24-hour dietary recalls), and quality of life (RAND Short Form-12).

Funding Sources: Brickhouse Greens