(P06-028-25) Measurement of Radical Scavenging and Cellular Metabolic Activity (CMA) of Plant-Based Food Matrices by Using EPR Technology

Objectives: To investigate the potential superoxide radical scavenging activities by a dietary supplement S7 and its ingredients

Methods: The EPR based radical detection assay CMA (cellular metabolic activity) can be performed using Noxygen's VitaScreen, which allows the detection of ROS in biological as well as ex-vivo samples using a spin probe CMH (1-hydroxy-3-methoxy-carbonyl-2,5,5-tetramethylpyrrolidine.HCl).

Results: The results suggested that S7 blend showed significant scavenging activity of superoxide in concentration dependent manner up to 70% with the concentration range 0-0.5 µg/mL. Amongst the ingredients green tea extract showed strongest scavenging activity upto 80 % in the tested concentrations. Effect of S7 on cellular “total” ROS generation/ metabolic activity in human participants also investigated and resulted significant reduction in CMA.

Conclusions: S7 blend and it's ingredients viz. green tea extract (46.5%), green coffee extract (46.5%), turmeric (5%), blueberry (0.5%), tart cherry (0.5%), broccoli (0.5) and kale extract (0.5%) showed significant scavenging activity of superoxide in concentration dependent manner. This study of screening food products and its ingredients is expected to guide to develop natural dietary plant products or supplement that could be effective in modulation of oxidative stress.

Funding Sources: N/A