(P06-030-25) Effect of Perilla Oil on Blood Coagulation Using the PhenFlex Challenge Test in Healthy Smokers: A Randomized, Double-Blind, Placebo-Controlled, Parallel Trial

Objectives: Smokers have been shown to exhibit increased lipid peroxidation, oxidative stress, and immune system imbalance, with spontaneous platelet aggregation and increased platelet activation during thrombus formation observed in their plasma. Additionally, cardiovascular lesions have been reported more frequently in current smokers than in nonsmokers. This study investigated the effects of Perilla oil (PO) on endothelial dysfunction induced by the PhenFlex challenge test (PFT) in healthy smokers.

Methods: In a randomized, double-blind, placebo-controlled parallel trial, 80 healthy smokers were randomly assigned to the placebo and test groups and received capsulated corn oil and PO (4.8 g/day) for 8 weeks, respectively. Blood samples were collected at week 0 and after 8 weeks. The PFT was performed at week 8, followed by additional blood sampling at 0, 120, 240, and 480 min. The closure times, including collagen epinephrine, collagen adenosine diphosphate, prothrombin, and activated partial thromboplastin, as well as the mRNA expression of inflammatory factors such as interleukin-6, tumor necrosis factor (TNF)-α, interferon-γ, and TBX21, were measured.

Results: During the 8 weeks, the collagen adenosine diphosphate closure time was delayed in the PO group compared to the placebo group (p = 0.094); however, there was no significant difference in the other closure times. In terms of TNF-α and TBX21, the PO group showed a significant decrease compared to the placebo group (p = 0.004 and 0.026, respectively). After the PFT, there were no significant differences in closure times between the two groups. However, the mRNA expression of TBX21 was significantly decreased in the PO group compared to the placebo group (p = 0.044).

Conclusions: In conclusion, consumption of PO may prevent inflammatory responses as well as blood coagulation-induced oxidative stress, thereby potentially suppressing the progression of thrombus formation.

Funding Sources: This research was supported by '2020 Discovering Functional Crops Project' of The Food Industry Promotional Agency of Korea, Korea and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT, and This research was supported by Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (20220488).