(P20-019-25) Enhancing Post-Operative Nutrition: Leveraging Fourier Transform Infrared Spectroscopy for Early Detection of Lipid Profile Changes After Roux-en-Y Gastric Bypass

Objectives: This study investigates the application of FTIR to identify early biochemical changes, particularly in lipid profiles, in individuals undergoing Roux-en-Y gastric bypass (RYGB).

Methods: Observational study with patients from a university hospital's Bariatric and Metabolic Surgery Program, with evaluations performed before (T0) and two months after (T1) RYGB. Biochemical parameters, anthropometric data, and body composition were assessed. FTIR spectra were pre-processed and analyzed using Principal Component Analysis and Partial Least Squares Discriminant Analysis. The normality of the data was evaluated using the Kolmogorov-Smirnov test, followed by paired T-tests or Wilcoxon tests as appropriate. Spearman correlation analysis of spectral information with biochemical parameters was also performed. A significance level of p< 0.05 was set for all tests. 

Results: The study evaluated 29 individuals (86.2% female), mean age of 41.2 ± 7.8 years. Significant differences were observed in anthropometric parameters and body composition (p < 0.001). Early improvements in the lipid profile were noted, with significant decreases in triglycerides, total cholesterol, and LDL cholesterol (p < 0.05) just two months post-surgery. FTIR identified correlations between biochemical parameters and specific spectral regions at T0 and T1. Serum triglycerides showed a significant correlation with the lipid-specific spectral region (1796–1685 cm^-1) at both time points (p < 0.05).

Conclusions: FTIR can effectively monitor biochemical changes in RYGB surgery patients. The spectral range associated with lipid functional groups (1796-1675 cm⁻¹) showed a significant relationship with serum triglyceride levels before and after RYGB. Additionally, various biochemical parameters exhibited strong correlations with other spectral regions, implying that the serum spectral profile can indicate biochemical variations at different post-surgery stages.

Funding Sources: Espírito Santo Research and Innovation Support Foundation, (FAPES)