(P17-064-25) Dietary Biotin Mitigates the Risk of Gastritis and Duodenitis: A Large-Scale Prospective Cohort Study and Mechanistic Exploration

Objectives: The impact of dietary biotin on gastritis and duodenitis (GD) remains unclear. This study investigated the association between dietary biotin and the risk of incident GD, and preliminarily explored potential underlying mechanisms.

Methods: Prospective data were derived from the UK Biobank, which included 203,221 participants without a history of GD who had completed at least one 24-hour dietary recall. Biotin intake was divided into low, moderate, and high groups based on tertiles. Time-varying Cox proportional-hazards models were employed to estimate the association between biotin intake and GD using hazard ratio (HR) and 95% confidence interval (CI), adjusted by demographics, lifestyle and other dietary vitamin B intake. Restricted cubic spline (RCS) Cox regression was used to investigate potential non-linear associations. Stratified analyses were performed by sex, age, energy intake and polygenic risk score (PRS). The mediating effects of blood biomarkers and chronic diseases were examined. Additionally, protein-protein interaction (PPI) network and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were further conducted.

Results: Compared with the low group, the moderate (HR=0.86[0.82,0.90]) and high (HR=0.83 [0.79,0.88]) group had lower risk of GD. A U-shaped curve was found, with the risk of GD bottoming out at 55 μg/day of dietary biotin. Similar results were found in stratified analysis by sex, energy intake and PRS. Higher level of biotin intake served as a higher protective factor significantly in younger. However, a similar protective effect was only observed at the moderate intake level in older individuals. The results also remained robust in sensitivity analyses. Lower incidences of gastroesophageal reflux disease, cardiovascular disease, chronic liver disease, and certain biomarkers were found to mediate the association. However, blood biomarkers for Helicobacter pylori were not found to have a mediating effect. On this basis, in combination with the results of PPI and KEGG, core genes such as EGFR, PTGS2, and MMP9 were identified, which affect cell proliferation, apoptosis, and inflammation.

Conclusions: Our population-based study first revealed that dietary biotin plays a protective role in GD, which may act through cell life cycle and systemic inflammation.

Funding Sources: None